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Part:BBa_K1967000:Design

Designed by: Brendan Largey   Group: iGEM16_Edinburgh_UG   (2016-10-15)


0_AB BabbleBrick (1.5)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Many considerations were taken into account during the design of BabbleBricks. The 1.5 format version has special considerations to make sure that there is a stop codon present in each from of every BabbleBrick to help prevent unwanted protein formation. Additionally, gap regions were placed throughout the BabbleBrick to prevent the formation of restriction sites. Cut sites illegal in PhytoBricks and BioBricks are also illegal to have in BabbleBricks, however it is more difficult to control their appearance because every BabbleBrick has a unique word coding and orc region. By introducing gap sequences, we have greater control over the whole sequence and can prevent any unwanted sites from forming.

For more information on the format and evolution of the BabbleBrick, see our wiki page on design: http://2016.igem.org/Team:Edinburgh_UG/Design


Source

Designed de novo by Edinburgh UG iGEM 2016

References